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Copyright © 2007 by Karl Oberholser,
Messiah College, Grantham PA USA.
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Common Structural Feature of Globular Proteins
These slides contain illustrations of:
^. Circular Layers
^.Five Layers
^.Non-stablizing Layers

Return to Two & Three Layer Examples

Made 2007 by KMO, Messiah College

2 Next Previous 1 3 4 5 6 7 8 9 10 View 8TIM in ProteinExplorer.Org

Circular Layers
Triose Phosphate Isomerase

The circular layers formed by the b sheet barrel and a helix barrel are clearly seen in this view, giving what would appear to be two layers.

Made 2007 by KMO, Messiah College

3 Next Previous 1 2 4 5 6 7 8 9 10 View 8TIM in ProteinExplorer.Org

Circular Layers
Hydrophobic residues

This view shows that hydrophobic residues occupy the central circular cavity as well as the space between the two circular layers. With this being the case one could say that the isomerase had four layers of backbone.

Made 2007 by KMO, Messiah College

4 Next Previous 1 2 3 5 6 7 8 9 10 View 8TIM in ProteinExplorer.Org

Circular Layers
Polar residues

Upon close inspection one will see that there are few polar residues within the central cavity and between the two circular layers.

Made 2007 by KMO, Messiah College

5 Next Previous 1 2 3 4 6 7 8 9 10 View 1ABB in ProteinExplorer.Org

Five Layers
Glycogen phosphorylase (Domain 2)

Rotate the structure and attempt to identify the five layers.

Made 2007 by KMO, Messiah College

6 Next Previous 1 2 3 4 5 7 8 9 10 View 1ABB in ProteinExplorer.Org

Five Layers
The five layers are shown in colors Brown through Red.

Made 2007 by KMO, Messiah College

7 Next Previous 1 2 3 4 5 6 8 9 10 View 1ABB in ProteinExplorer.Org

Five Layers
Hydrophobic residues

Compare the location of the balls in this slide with those in the next. The differences in location is not as obvious as with the previous proteins, but the hydrophobic residues tend to be between the layers, and the polar ones are on the surface (next slide).

Made 2007 by KMO, Messiah College

8 Next Previous 1 2 3 4 5 6 7 9 10 View 1ABB in ProteinExplorer.Org

Five Layers
Polar residues

Made 2007 by KMO, Messiah College

9 Next Previous 1 2 3 4 5 6 7 8 10 View 2BP2 in ProteinExplorer.Org

Non-stablizing Layers
Phospholipase A₂

Even though there are layers of backbone in this molecular structure, the presence of the layers by themselves do not make a major contribution to the stablity because there is not a significant interaction between the side chains of the layers, as seen in the next slide.

Made 2007 by KMO, Messiah College

10 LAST VIEW Previous Top 1 2 3 4 5 6 7 8 9 View 2BP2 in ProteinExplorer.Org

Non-stablizing Layers
The backbone layers are not in a position to permit a significant interaction of the side chains from the two layers. The covalent Disulfide Bonds is the major force involved in maintaining the tertiary structure.

Made 2007 by KMO, Messiah College

Made with MolSlides.ProteinExplorer.Org

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